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KMID : 0358219940210020191
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Korean Journal of Fertility and Sterility
1994 Volume.21 No. 2 p.191 ~ p.200
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Effect of Hypoxanthine and Ovarian Steroids on the Maturation of Mouse Oocytes
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노효섭
정영주/조한구/박환규/송완례/이기숙/김종덕
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Abstract
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The influence of hypoxanthine and ovarian steroids on the meiotic maturation process of mouse oocytes was investigated for the qualified application of culture medium in in vitro fertilization (IVF). Mouse oocytes were cultured in hypoxanthine
and
various ovarian steroids (progesterone, estradiol-17β and testosterone) and their effects on the oocyte maturation had been observed. When mouse oocytes were cultured in the various concentration (1-4mM) of hypoxanthine, meiotic maturation of
cumulus
cell-enclosed oocytes was inhibited by presence itself, which was a dose-dependent effect in meiotic arrest of mouse oocytes. The presence of progesterone,extradiol-17β and testosterone have made the mouse oocyte mature properly. Meanwhile
maturation
of cumulus cell-enclosed oocyte was severely inhibited by 3 hours-culture in the media of progesterone supplemented with hypoxanthine. However the continuous presence lasting 24 hours of progesterone even supplemented with hypoxanthine had got
rid
of
the inhibition of oocytes maturation. Not only estradiol-17β supplemented with hypoxanthine but also testosterone supplemented with hypoxanthine exert the severe inhibition of the maturation of cumulus cell-enclosed oocytes for 3-hours culture.
However
the continuous presence lasting24 hours of estradiol-17β and testosterone even supplemented with hypoxanthine had relieved the inhibition of oocytes maturation.
These results make us suggest that hypoxanthine inhibits the mouse oocyte maturation, particularly markedly in conjunction with ovarian steroids for short period, which indicated some sort of the synergistic inhibitory retationship between the
ovarian
steroids and hypoxanthine.
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